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Title: If the fragment of DNA shown below were to replicate, on which strand (A or B) would Okazaki fragments be formed?
If the fragment of DNA shown below were to replicate, on which strand (A or B) would Okazaki fragments be formed?
The origin of replication is at the right and the replication fork proceeds towards the left. Explain your reasoning.
Strand A: 5 -ATCGATCCCTAG-3
Strand B: 3 -TAGCTAGGGATC-5
Answer:
Strand A. (answer)
The nascent strand being synthesized using Strand A as template is the lagging strand, and hence would have discontinuous synthesis with okazaki fragments.
The leading strand synthesis proceeds in the direction of the replication fork, and is synthesized 5' first and proceeds 3'-ward. In your example, the leading strand is using strand B as template.
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